Hello,

If your low E/6th string is not in tune while manually tuning your guitar, it can adversely affect your overall sound quality and playability. A poorly tuned string can lead to dissonance in chords, inaccurate melodies, and hinder your playing experience. To address this, follow these steps:

Tension Adjustment: Begin by gently turning the tuning peg for the low E string in the appropriate direction. Tune either up (tightening the string) or down (loosening the string) depending on whether the pitch is too high or low.

Reference Pitch: If you have access to a reference pitch (e.g., a piano or digital tuner), compare the string's sound to the correct pitch. Adjust the tension gradually until both pitches match.

Use Harmonics: Alternatively, you can tune the low E string to harmonics on the 7th fret of the A string. Adjust the low E string's tension until the harmonics on the 7th fret of the low E string matches those on the 5th fret of the A string.

Fine-tuning: Once you're close to the correct pitch, pluck the low E string and listen carefully. Make small adjustments to the tension for precise tuning. Recheck and Retune: After adjusting, go through the tuning process again to ensure accuracy.